streptomyces avermitilis中文什么意思

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  1. Effect of avec gene deletion on avermectins production in streptomyces avermitilis
    基因缺失对产素调控的影响
  2. The transformants were cultivated and the harvested cells were used in the procedure of conjugation by which the recombinant plasmid pid03 and pc05 were introduced into streptomyces avermitilis s - 2 respectively
    经过在mym平板上的传代和抗性标记的筛选分别得到了同源双交换的菌株aved24和avecg 。
  3. In order to obtain a dna fragment containing aved gene , 5 ' end primer and 3 ' end primer , consisting of 18 and 20 nucleotides respectively , were designed and synthesized . a 2 . 2 kb dna fragment containing aved gene was obtained by pcr when genomic dna of streptomyces avermitilis s - 2 was used as a template
    为了得到aved基因片段,首先根据已知的阿维菌素生物合成基因簇的核苷酸序列,设计并合成了由18和20个核苷酸组成的5端和3端引物;以streptomycesavermitiliss - 2基因组dna为模板,经pcr扩增得到了2 . 2kb的含有aved基因的dna片段。
  4. In addition to avermectins , s . avermitilis produces oligomycin , a strongly toxic compound . gene deletion vector pxl05 was used to disrupt oligomycin polyketide synthase ( pks ) encoding genes ( olma ) in streptomyces avermitilis cz8 - 73 , the producer of anthelmintic avermectins b and the cell growth inhibitor oligomycin . olma gene cluster in the chromosome was displaced by deletion allele on the plasmid via double crossover
    本研究以产阿维菌素b和寡霉素的阿维链霉菌cz8 - 73为出发菌株,构建了基因缺失载体pxl05 ,并将其转入cz8 - 73中,通过缺失载体和染色体之间的同源双交换,对染色体上长达90kb的寡霉素聚酮合酶( pks )基因簇( olma )进行了缺失。
  5. In this study , the avermectin - producing strain streptomyces avermitilis was studied and the avermectin biosynthesis gene cluster in the genomic dna of streptomyces avermitilis s - 2 was altered by the method of gene engineering . insertion inactivation of aved gene in the cluster by introducing apramycin resistance gene into aved gene resulted in the disappearance of " a " components of avermectins . when avec gene was inactivated by the same way , four " 1 " components were lost and only " 2 " components , the potential precursor of ivermectin , were accumulated
    将该基因簇中的aved基因通过插入外源的安普霉素抗性基因片段使其失活,导致发酵产物中4个a组分(不需要的组分)的消失;将基因簇中的avec基因通过同样手段,使其失活,导致发酵产物中4个“ 1 ”组分的消失,而主要积累“ 2 ”组分(进一步改造可成为伊维菌素的前体b _ 2组分) 。

百科释义

et al. 1979) Kim and Goodfellow 2002
详细百科解释

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